The method to visualize and localize proteins in cells. The detection can be direct with a primary antibody coupled to a reporter (fluorescent dye, enzyme, etc.) or, to increase the sensitivity, indirect. This second approach is the most used. Indeed the multiple binding of secondary antibodies to a first antibody directed against the protein of interest will amplify the signal and allow a detection with higher sensitivity.
Antibody-Adviser is a non-profit association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. At Antibody-Adviser, we aim to make scientist's life easy and we are really proud to improve the laboratory experience! Antibody-Adviser was created in 2011 by a team of experts in Biology and Computer Science.