The method to visualize and locate proteins in tissues. The detection is based on the use of antibodies binding specifically to proteins expressed in the cells of a tissue. The popular indirect detection method involves two antibodies with a first antibody targeting the protein and then the second antibody directed against the primary antibody. This secondary antibody is conjugated to an enzyme that can produce colour or tagged with a fluorophore.
Antibody-Adviser is a non-profit association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. At Antibody-Adviser, we aim to make scientist's life easy and we are really proud to improve the laboratory experience! Antibody-Adviser was created in 2011 by a team of experts in Biology and Computer Science.