Antibody-Adviser: Keeping track of antibodies has never been so easy!


A widely used technique to detect proteins in extracts prepared from cells or tissues. Samples are run on a gel electrophoresis and proteins are then transferred to a membrane that will be incubated with primary and secondary antibodies. The horseradish peroxidase is the most common enzyme linked to the secondary antibodies. This enzyme cleaves a chemiluminescent agent and produces luminescence that will be capture by the visualization device. Not all western blot methods are quantitative but the selection of the good detection / visualization enables accurate protein quantification.


Antibody-Adviser is a non-profit association developing a unique platform to enable fast and efficient information sharing on any antibody used for research. At Antibody-Adviser, we aim to make scientist's life easy and we are really proud to improve the laboratory experience! Antibody-Adviser was created in 2011 by a team of experts in Biology and Computer Science. ©